The Dionex UltiMate 3000 is a modular High-Performance Liquid Chromatography (HPLC) and UHPLC system designed for versatile, robust, and high-performance liquid chromatography separation across analytical, micro, and semi-preparative scales.
Specifications
General
| Parameter | Value |
|---|---|
| Interfaces | USB 2.0 (Type-B for PC connection; integrated 3-port Type-A hub for system inter-module communication) |
| Display | 2-line × 20-character LCD display with status LEDs per standard module |
Physical
| Parameter | Value |
|---|---|
| Power Supply | 100–240 V AC, 50/60 Hz, automatic voltage selection |
| Operating Temperature | 10 to 35 °C |
| Dimensions (W×H×D) | 361 mm × 165 to 200 mm × 578 mm (typical per individual module, height varies) |
| Weight | 10 - 22 kg (module dependent) |
Solvent Delivery System (Pump)
| Parameter | Value |
|---|---|
| Pump Configurations | Isocratic, Binary (HPG), Quaternary (LPG), or Dual-Gradient (DGP) |
| Flow Rate Range | 0.001 to 10 mL/min (Analytical SD/RS), up to 100 mL/min (Semiprep), 10 nL/min to 50 µL/min (Nano/Capillary) |
| Flow Rate Accuracy | ±0.1% |
| Flow Rate Precision | < 0.05% RSD |
| Maximum Operating Pressure | 620 bar (SD series) / 1034 bar (RS series) |
| Composition Range | 0 to 100% in 0.1% steps |
| Composition Precision | < 0.15% SD |
| System Delay Volume | Standard 400 µL (configurable down to 25 µL for capillary/nano) |
Autosampler & Injection System
| Parameter | Value |
|---|---|
| Injection Principle | Split-loop (in-needle) |
| Sample Capacity | Up to 384-well plates, up to 120 × 2 mL vials (configuration dependent) |
| Sample Thermostatting Temperature Range | 4 °C to 45 °C (thermostatted WPS models) |
| Injection Cycle Time | < 15 s (with standard draw speed) |
| Carryover | < 0.004% (with external needle wash) |
Column Compartment / Thermostat
| Parameter | Value |
|---|---|
| Temperature Control Method | Thermoelectric (Peltier) heating and cooling |
| Temperature Range | 5 °C to 80 °C (TCC-3000SD) / 5 °C to 110 °C (TCC-3000RS) |
| Temperature Stability | ±0.1 °C |
| Temperature Accuracy | ±0.5 °C |
| Pre-Column Heating | Passive or active pre-column eluent heaters supported |
Detector Options & Performance
| Parameter | Value |
|---|---|
| Supported Detector Types | Diode Array (DAD), Multi-Wavelength (MWD), Variable Wavelength (VWD), Fluorescence (FLD), Charged Aerosol (CAD), Refractive Index (RID) |
| Wavelength Range (DAD) | 190 to 800 nm (DAD-3000), 190 to 1000 nm (DAD-3000RS) |
| Data Acquisition Rate | Up to 100 Hz (DAD-3000RS), up to 200 Hz (VWD-3400RS) |
| Wavelength Accuracy | ±1 nm |
System Fluidics & Materials
| Parameter | Value |
|---|---|
| Wetted Materials | Stainless steel, PEEK, FEP, PTFE, sapphire, ruby, ceramic, titanium (configuration dependent) |
| pH Range Support | pH 1 to 13 (standard stainless steel), pH 1 to 14 (biocompatible systems) |
| Biocompatible Flow Path | Yes, available with BioRS / biocompatible configurations |
Software & Control
| Parameter | Value |
|---|---|
| Supported Chromatography Data Systems | Thermo Scientific Chromeleon CDS (native); driver integration available for third-party CDS |
| Compliance Capability | 21 CFR Part 11 compliant when operated via Chromeleon CDS |
| GLP & Compliance Features | System wellness monitoring, qualification wizards, comprehensive audit trail, GLP history |
Physical & Utility Requirements
| Parameter | Value |
|---|---|
| Module Width | 361 mm |
| Module Depth | 578 mm |
| Module Height | 165 mm (standard pump/detector), 200 mm (autosampler) |
| Leak Detection | Active optical leak sensors with individual module drainage channels |
Environmental & Safety Compliance
| Parameter | Value |
|---|---|
| Operating Humidity Range | 20% to 80% relative humidity (non-condensing) |
| Overpressure Protection | Automatic pump shutdown upon exceeding user-defined or factory pressure limits |
Fluidic Connections
| Parameter | Value |
|---|---|
| Capillary Fitting System | Viper fingertight fitting system (zero-dead-volume, rated up to 1250 bar) |
Pump Performance
| Parameter | Value |
|---|---|
| Pumping Technology | SmartFlow technology for pulsation-free solvent delivery and automatic compressibility compensation |
| Mixing Technology | SpinFlow mixing design using interchangeable radial and longitudinal mixing chambers |
Detector Specifications
| Parameter | Value |
|---|---|
| Detector Flow Cell Technology | LightPipe flow cells (available for select detectors, offering minimized dispersion and long path lengths) |
Maintenance & Diagnostics
| Parameter | Value |
|---|---|
| System Diagnostic Features | System Wellness monitoring for active components, including piston seal wear and lamp lifetime tracking |
Autosampler
| Parameter | Value |
|---|---|
| Injection Volume Precision | < 0.25% RSD (typically < 0.15% at 20 µL) |
Column Thermostat
| Parameter | Value |
|---|---|
| Column Oven Temperature Control | Peltier heating and cooling |
| Column Identification | Radio-frequency identification (RFID) chip tracking |
Pump
| Parameter | Value |
|---|---|
| Piston Seal Wash | Active wash system (fully integrated/automated) |
Interfaces
| Parameter | Value |
|---|---|
| Digital I/O | 2 digital inputs, 2 relay outputs (module-specific) |
Technology
High-Performance Liquid Chromatography (HPLC) & UHPLC
Applications
Liquid Chromatography Separation, Analytical and Preparative Chemistry, Pharmaceutical Analysis
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