The Eppendorf Mastercycler (often referred to as the classic Mastercycler, model 5333) is a foundational Peltier-based thermal cycler designed for standard Polymerase Chain Reaction (PCR) applications. It is renowned for its iconic universal block that accommodates 0.2 mL tubes, 0.5 mL tubes, or a 96-well PCR plate without requiring block changes. It features a heated lid, internal memory for up to 100 programs, and expansion via the Eppendorf Personal Card.
Specifications
General
| Parameter | Value |
|---|---|
| Interfaces | RS-232 C, Centronics (printer) |
Physical
| Parameter | Value |
|---|---|
| Power Supply | 115 V or 230 V, 50/60 Hz; 500 W max |
| Dimensions (W×H×D) | 260 × 270 × 410 |
| Weight | 10 |
Sample Block Specifications
| Parameter | Value |
|---|---|
| Block Capacity | 96 × 0.2 mL tubes, 77 × 0.5 mL tubes, or 1 × 96-well PCR plate |
Thermal Performance
| Parameter | Value |
|---|---|
| Block Temperature Range | 4 °C to 99 °C |
| Max Heating Rate | Up to 3 °C/s |
| Max Cooling Rate | Up to 2 °C/s |
Software & Programming
| Parameter | Value |
|---|---|
| Max Number of Programs | 100 (internal memory) |
| Memory Expansion | Eppendorf Personal Card (Smart Card) |
Power & Electrical
| Parameter | Value |
|---|---|
| Max Power Consumption | 500 W |
Technology
Peltier-based thermal cycling
Applications
Polymerase Chain Reaction (PCR)