The Waters ACQUITY UPLC is a pioneering Ultra-Performance Liquid Chromatography (UPLC) system designed to deliver superior resolution, sensitivity, and speed compared to traditional HPLC. Utilizing sub-2-micron particle columns and capable of operating at up to 15,000 psi, the system includes modules such as the Binary Solvent Manager (BSM) and Sample Manager (SM). It is widely used in pharmaceutical, chemical, and biological laboratories for complex mixture analysis.
Specifications
System General Specifications
| Parameter | Value |
|---|---|
| System Type | Ultra-Performance Liquid Chromatography (UPLC) |
| Maximum Operating Pressure | 15,000 psi (1034 bar) |
| Operating Flow Rate Range | 0.010 to 2.000 mL/min |
| pH Range | 2 to 12 |
| System Delay Volume | < 120 µL (standard configuration) |
Solvent Management (Pump)
| Parameter | Value |
|---|---|
| Number of Solvents | Up to 4 (A1/A2, B1/B2 selection) |
| Solvent Blending/Mixing Type | High-pressure binary mixing |
| Integrated Vacuum Degassing | Yes |
| Flow Rate Precision | 0.075% RSD or 0.02 min SD (whichever is greater) |
| Composition Accuracy | ± 0.5% absolute (from 5% to 95%) |
| Compressibility Compensation | Automatic, continuous |
| Plunger Seal Wash | Integral, active, programmable |
| Gradient Profiles | 11 (including linear, step, concave, convex) |
| Wet Priming | Automated |
Sample Management (Autosampler)
| Parameter | Value |
|---|---|
| Injection Volume Range | 0.1 to 50.0 µL standard |
| Vial/Plate Formats Supported | 96-well and 384-well plates, 48-position 2-mL vial trays |
| Sample Temperature Range | 4.0 to 40.0 °C |
| Sample Carryover | < 0.005% (with standard wash configurations) |
| Needle Wash System | Strong and weak solvent dual wash |
Column Management
| Parameter | Value |
|---|---|
| Column Temperature Range | 20.0 to 90.0 °C (with High Temperature Column Heater) |
| Column Tracking Technology | eCord Technology |
Detector Compatibility & Integration
| Parameter | Value |
|---|---|
| Supported Optical Detectors | Tunable UV (TUV), Photodiode Array (PDA), Fluorescence (FLR), Refractive Index (RI), Evaporative Light Scattering (ELS) |
| Supported Mass Spectrometry Detectors | SQD, TQD, QDa, Xevo series, SYNAPT series |
Software and Control
| Parameter | Value |
|---|---|
| Chromatography Data System (CDS) Compatibility | Empower, MassLynx |
Sample Management
| Parameter | Value |
|---|---|
| Base Sample Capacity | 2 microtiter plates (96 or 384-well) or up to 96 vials (2 mL) |
| Expanded Sample Capacity | Up to 22 microtiter plates with optional Sample Organizer |
| Injection Modes | Full loop, partial loop, and partial loop with needle overfill |
| Injection Linearity | > 0.999 coefficient of deviation |
Technology
Ultra-Performance Liquid Chromatography (UPLC)
Applications
Analytical Chemistry, Chromatography